Bukayo Saka is out of England’s Nations League game against Finland on Sunday.
The winger limped off during the second half of Thursday’s humiliating defeat to Greece at Wembley and is not fit for the match in Helsinki.
He will now return to Arsenal for further assessment ahead of their Premier League clash with Bournemouth next weekend.
Liverpool midfielder Curtis Jones is also out of the trip as he has a personal matter to deal with.
Jones was a late addition to the England squad ahead of the Greece game but was an unused substitute.
Harry Kane was one of 22 players who trained on Saturday, increasing hopes that the captain will be fit after missing Thursday’s game.
Kane could not overcome a knock picked up playing for Bayern Munich in time to feature and his absence prompted interim boss Lee Carsley to deploy an eyebrow-raising formation, which had Jude Bellingham playing a “false 9” in front of Phil Foden, Saka, Cole Palmer and Anthony Gordon.
It backfired as Greece won 2-1 to take control of League B Group 2, leaving England no room for any more slip-ups if they want to win the group and return to League A automatically.
Meanwhile, England goalkeeper Jordan Pickford says Carsley will be hurt by the humiliating loss to Greece, but does not know how the interim manager’s future will be affected.
The former Republic of Ireland international Carsley was cryptic about whether he wants the job full-time after the game, yet that might be out of his control after a bold attacking selection backfired.
The defence was left woefully exposed and the visitors, mourning the death of teammate George Baldock, took advantage.
They were worthy winners, with Vangelis Pavlidis scoring a stoppage-time decider.
Asked how the defeat might affect Carsley’s future, Pickford said: “I’m not the hierarchy. It’s never nice for a player or staff to get beat at Wembley.
“We want to win every game we play when we put this shirt on and we didn’t.
“So we’ve got to keep going, dust ourselves down. What can we do better, what can we learn from and move on and get the result on Sunday?
“You’ve always got to learn. You’re never going to be perfect, even when you’re winning games. You can learn a lot from this. It’s about recovering well and getting ready for Sunday.”
Pickford hopes skipper Kane will be available. “He’s not the record goalscorer for England and has 100 caps for nothing, he leads by example,” he said.
“I think Harry will be fit to go on Sunday. Now we’ve just got to go and win away on Sunday against Finland and then the next camp in Greece away and Ireland at home.”
England must now win their remaining three games, starting with Finland on Sunday before the return trip to Athens and a Wembley fixture against the Republic in the November fixtures.
